Synaptic Systems - VGluT1 sdAb

Cat. No.: N1602-At488-L

Amount: 200 µl

Price: $515.00

Cat. No. N1602-At488-L	200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO^® 488. Albumin was added for stabilization. For reconstitution add 200 µl H₂O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	IP: N/A ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet
Label	ATTO 488, two fluorophores coupled to one FluoTag
Clone	Nb9
Subtype	single domain
Immunogen	Recombinant protein corresponding to AA 58 to 515 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity	Reacts with: rat (Q62634), mouse (Q3TXX4). Other species not tested yet.

References for VGluT1 sdAb - N1602-At488-L

Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport.
Schenck S, Kunz L, Sahlender D, Pardon E, Geertsma ER, Savtchouk I, Suzuki T, Neldner Y, Štefanić S, Steyaert J, Volterra A, et al.
Biochemistry (2017) 5630: 3962-3971. N1602-At488-L ICC; tested species: mouse

Cat. No.: N1602-At488-L

Amount: 200 µl

Price: $515.00

ICC

IHC

Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

1 ) Takamori S et al., Nature (2000)
2 ) Bellocchio EE et al., Science (2000)
3 ) Bellocchio EE et al., J. Neurosci. (1998)
4 ) Ni B et al., Proc. Natl. Acad. Sci. U.S.A. (1994)

Protocols

Other products for this target

VGluT1 sdAb - FluoTag-X2 - N1602-At488-L

This product was developed by

References for VGluT1 sdAb - N1602-At488-L